Some useful numbers for plasmid DNA extraction

plasmid_dna

GFP plasmid DNA 뽑으면서 궁금했던 것들을 찾아서 정리해봤다.

Optimal Optical Density at 600nm (OD600) for plasmid DNA extraction = 2~4
– This is the log growth phase

OD600 = 1 → 1.6 x 10 ^ 9 cells / mL
– correlation of cell count and optical density

1 x 10 ^ 8 cells → 500ng DNA
– correlation of cell count and estimated DNA yield

그래서!
1.5mg binding capacity를 가진 내가 사용하는 Plasmid DNA extraction kit에서는
OD600 = 2 정도의 대장균 200mL을 사용하면 cell count가 3 x 10 ^ 11이 나오고,
Loss를 고려하면 여기서 DNA를 뽑으면 딱 맞을 것 같다.
즉, 너무 뿌옇게 되기 전에 large culture를 끊어주는 것이 좋겠다.

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